69. Byun, S., Park, C., Suh, J.-Y., Witte, C.-P., and Rhee, S. (2024) Structure, cooperativity, and inhibition of the inosine 5’-monophosphate-specific phosphatase from Saccharomyces cerevisiae. FEBS J. 291(9), 1992-2008.
68. Lee, J., Kim, S.-H., and Rhee, S. (2024) Structure and function of the pseudouridine 5’-monophosphate glycosylase PUMY from Arabidopsis thaliana. RNA Biol. 21(1), 1-10.
67. Chen, X., Kim, S.-H., Rhee, S., and Witte, C.-P. (2023) A plastid nucleoside kinase is involved in inosine salvage and control of purine nucleotide biosynthesis. Plant Cell 35(1), 510-528.
66. Choi, M., and Rhee, S. (2022) Structural and biochemical basis for the substrate specificity of Pad-1, an indole-3-pyruvic acid aminotransferase in auxin homeostasis. J. Struct. Biol. 214(2), 107857
65. Kim, S.-H., Kim, M., Park, D., Byun, S., and Rhee, S. (2022) Substrate-binding loop interactions with pseudouridine trigger conformational changes that promote catalytic efficiency of pseudouridine kinase PUKI. J. Biol. Chem. 298(5), 101869
64. Heinemann, K., Yang, S.-Y., Straube, H., Medina-Escobar, N., Varbanova-Herde, M., Herde, M., Rhee, S.*, and Witte, C.-P.* (2021) Initiation of cytosolic plant purine catabolism involves a monospecific xanthosine monophosphate phosphatase. Nat. Commun. 12, 6846
63. Kim, S.-H., Park, S., Park, E., Kim, J.-H., Ghatge, S., Hur, H.-G., and Rhee, S. (2021) Structure and substrate specificity determinants of NfnB, a dinitroaniline herbicide-catabolizing nitroreductase from Sphingopyxis sp. strain HMH. J. Biol. Chem. 297(4), 101143
62. Kim, S.-H., Witte, C.-P., and Rhee, S. (2021) Structural basis for the substrate specificity and catalytic features of pseudouridine kinase from Arabidopsis thaliana. Nucleic Acids Res. 49(1), 491-503.
61. Jin, S.-H., Lee, H., Shin, Y., Kim, J.-H., and Rhee, S. (2020) Crystal structure of the indole-3-acetic acid-catabolizing enzyme DAO1 from Arabidopsis thaliana. J. Struct. Biol. 212(3), 107632
60. Kim, S.-H., Khan, R., Choi, K., Lee, S.-W.*, and Rhee, S.* (2020) A triclosan-resistance protein from the soil metagenome is a novel enoyl-acyl carrier protein reductase: Structure-guided functional analysis. FEBS J. 287(21), 4710-4728.
59. Lee, H., and Rhee, S. (2020) Structural and mutational analyses of the bifunctional arginine dihydrolase and ornithine cyclodeaminase AgrE from the cyanobacterium Anabaena. J. Biol. Chem. 295(17), 5751-5760.
58. Kang, P.-A, Oh, J., Lee, H., Witte, C.P., and Rhee, S. (2019) Crystal structure and mutational analyses of ribokinase from Arabidopsis thaliana. J. Struct. Biol. 206(1), 110-118.
57. Kim. S.-H., Kang, P.-A, Han, K., Lee, S.-W.*, and Rhee, S*. (2019) Crystal structure of chloramphenicol-metabolizing enzyme EstDL136 from a metagenome. PLoS ONE 14(1), e0210298
56. Oh, J., Liuzzi, A., Ronda, L., Marchetti, M., Corsini, R., Folli, C., Bettani, S., Rhee, S.* and Percudani, R.* (2018) Diatom allantoin synthase provides structural insights into natural fusion protein therapeutics. ACS Chem. Biol. 13(8), 2237-2246.
55. Oh, J., Lee, D.-G., Bahn, Y.-S., and Rhee, S. (2017) Crystal structure of inositol 1,3,4,5,6-pentakisphosphate 2-kinase from Cryptococcus neoformans. J. Struct. Biol. 200(2), 118-123.
54. Oh, J., Hwang, I., and Rhee, S. (2016) Structural insights into an oxalate-producing serine hydrolase with an unusual oxyanion hole and additional lyase activity. J. Biol. Chem. 291(29), 15185-15195.
53. Park, Y., Cho, Y., Lee, Y.H., Lee, Y.W., and Rhee, S. (2016) Crystal structure and functional analysis of isocitrate lyases from Magnaporthe oryzae and Fusarium graminearum. J. Struct. Biol.194(3), 395-403.
52. Park, AR., Son, H., Min, K., Park, J., Goo, JH., Rhee, S., Chae, SK., and Lee, YW. (2015) Autoregulation of ZEB2 expression for zearalenone production in Fusarium graminearum. Mol. Microbiol. 97(5), 942-956.
51. Park, Y., Shin, I., and Rhee, S. (2015) Crystal structure of the effector protein HopA1 from Pseudomonas syringae. J. Struct. Biol. 189(3), 276-280
50. Han, M., Park, Y., Kim, I., Kim, E.-H., Yu, T.-K., Rhee, S.* and Suh, J.-Y.* (2014) Structural basis for the auxin-induced transcriptional regulation by Aux/IAA17. Proc. Natl. Acad. Sci. USA 111(52), 18613-18618.
49. Yu, S., Hwang, I., and Rhee, S. (2014) The crystal structure of type III effector protein XopQ from Xanthomonas oryzae complexed with adenosine diphosphate ribose. Proteins 82, 2910-2914.
48. Shin, I., Han, K., and Rhee, S. (2014) Structural insights into the substrate specificity of (S)-ureidoglycolate amidohydrolase and its comparison with allantoate amidohydrolase. J. Mol. Biol. 426(17), 3028-3040.
47. Oh, J., Goo, E., Hwang, I., and Rhee, S. (2014) Structural basis for bacterial quorum sensing-mediated oxalogenesis. J. Biol. Chem. 289(16), 11465-11475.
46. Park, Y., Lim, J.A., Kong, M., Ryu, S., and Rhee, S. (2014) Structure of bacteriophage SPN1S endolysin reveals an unusual two-module fold for the peptidoglycan lytic and binding activity. Mol. Microbiol. 92(2), 316-325.
45. Puggioni, V., Dondi, A., Folli, C., Shin, I., Rhee, S., and Percudani, R. (2014) Gene context analysis reveals functional divergence between hypothetically equivalent enzymes of the purine-ureide pathway. Biochemistry. 53(4), 735-745.
44. Yu, S., Hwang, I., and Rhee, S.. (2013) Crystal structure of the effector protein XOO4466 from Xanthomonas oryzae. J. Struct. Biol. 184(2), 361-366
43. Park, J., and Rhee, S. (2013) Structural basis for a cofactor-dependent oxidation protection and catalysis of cyanobacterial succinic semialdehyde dehydrogenase. J. Biol. Chem. 288, 15760-15770.
42. Lee, H.Y., Seo, J.S., Cho, J.H., Jung, H., Kim, J.K., Lee, J.S., Rhee, S.*, and Choi, Y.D.* (2013) Oryza sativa COI homologues restore jasmonate signal transduction in Arabidopsis coi1-1 mutants. PLoS ONE 8(1): e52802. doi:10.1371/journal.pone.0052802.
41. Kim, M-I., Shin, I., Cho, S., Lee, J., and Rhee, S. (2012) Structural and functional insights into (S)-ureidoglycolate dehydrogenase, a metabolic branch point enzyme in nitrogen utilization. PLoS ONE 7(12): e52066. doi:10.1371/journal.pone.0052066.
40. Park, J., Kim, M. I., Park, Y. D., Shin, I., Cha, J., Kim, C. H., and Rhee, S. (2012) Structural and functional basis for substrate specificity and catalysis of levan fructotransferase. J. Biol. Chem. 287, 31233-31241.
39. Shin, I., Percudani, R., and Rhee, S. (2012) Structural and functional insights into (S)-ureidoglycine aminohydrolase, a key enzyme of purine catabolism in Arabidopsis thaliana. J. Biol. Chem. 287, 18796-187805.
38. Jung, W.-S., Lee, J., Kim, M.-I., Ma, J., Nagamatsu, T., Goo, E., Kim, H., Hwang, I., Han, J., and Rhee, S. (2011) Structural and Functional Analysis of Phytotoxin Toxoflavin-Degrading Enzyme. PLoS ONE 6, e22443. doi:10.1371/journal.pone.0022443
37. Chung, J., Goo, E., Yu, S., Choi, O., Lee, J., Kim, J., Kim, H., Igarashi, J., Suga, H., Moon, J.S., Hwang, I., and Rhee, S. (2011) Small-molecule inhibitor binding to an N-acyl-homoserine lactone synthase. Proc. Natl. Acad. Sci. USA 108, 12089-12094.
36. Yu, S., Lee, N. Y., Park, S.-J., and Rhee, S. (2011) Crystal structure of Toll-like receptor 2-activating lipoprotein IlpA from Vibrio vulnificus. Proteins 79, 1020-1025.
35. Roh, E., Park, T. H., Kim, M. I., Lee, S., Ryu. S,, Oh, C. S., Rhee, S., Kim, D. H., Park, B. S., and Heu, S. (2010) Characterization of a new bacteriocin, carocin D, from Pectobacterium carotovorum subsp. carotovorum Pcc21. Appl. Environ. Microbiol. 76, 7541-7549.
34. Kim, H, J., Yeom, S. J., Kim, K., Rhee, S., Kim, D., and Oh, D. K. (2010) Mutational analysis of the active site residues of a D-psicose 3-epimerase from Agrobacterium tumefaciens. Biotechnol. Lett. 32, 261-268.
33. Cho, J.-H., Jung, D.-K., Lee, K., and Rhee, S (2009) Crystal structure and functional analysis of the extradiol dioxygenase LapB from a long-chain alkylphenol degradation pathway in Pseudomonas. J. Biol. Chem. 284, 34321-34330.
32. Kim, K., Kim, M.-I., Chung, J., Ahn, J.-H., and Rhee, S. (2009) Crystal structure of metal-dependent allantoinase from Escherichia coli. J. Mol. Biol. 387, 1067-1074
31. Cho, J.-H., Park, Y., Ahn, J.-H., Lim, Y., and Rhee, S. (2008) Structural and functional insights into O-methyltransferase from Bacillus cereus. J. Mol. Biol. 382, 987-997.
30. Kim, M.-I., Kim, H.-S., Jung, J., and Rhee, S. (2008) Crystal structure and mutagenesis of sucrose hydrolase from Xanthomonas axonopodis pv. glycines: Insight into the exclusively hydrolytic amylosucrase fold. J. Mol. Biol. 380, 636-647.
29. Kim, K., Park, J., and Rhee, S. (2007) Structural and functional basis for (S)-allantoin formation in the ureide pathway. J. Biol. Chem. 282, 23457-23464.
28. Jung, C., Lyou, S. H., Yeu, S.Y., Kim, M. A., Rhee, S., Kim, M., Lee J. S., Choi, Y. D., and Cheong, J.-J. (2007) Microarray-based screening of jasmonate-responsive genes in Arabidopsis thaliana. Plant Cell Rep. 26, 1053-1063.
27. Oh, J., Kim, J.-K., Jeon, E., Yoo, C.-H., Moon, J.S., Rhee, S., and Hwang, I. (2007) Amyloidogenesis of type III-dependent harpins from plant pathogenic bacteria. J. Biol. Chem. 282, 13601-13609.
26. Jung, W.-S., Hong, C.-K., Lee, S., Kim, C.-S., Kim, S.-J., Kim, S.-I., and Rhee, S. (2007) Structural and functional insights into intramolecular fructosyl transfer by inulin fructotransferase. J. Biol. Chem. 282, 8414-8423.
25. Cho, J.-H., Lim, Y., Ahn, J.-H., and Rhee, S. (2007) Crystallization and preliminary X-ray diffraction analysis of BcOMT2 from Bacillus cereus: a family of O-methyltransferase. J. Microbiol. Biotechnol. 17, 369-372.
24. Lee, Y., Kim, K., Suh, J.-W., Rhee, S., and Lim, Y. (2007) Binding study of AfsK, a Ser/Thr kinase from Streptomyces coelicolor A3(2) and S-adenosyl-L-methionine. FEMS Microbiol. Lett. 266, 236-240.
23. Kim, K., Kim, H.-J., Oh, D.-K., Cha, S.-S., and Rhee, S. (2006) Crystal structure of D-psicose 3-epimerase from Agrobacterium tumefaciens and its complex with true substrate D-fructose: a pivotal role of metal in catalysis, an active site for the non-phosphorylated substrate, and its conformational changes. J. Mol. Biol. 361, 920-931.
22. Jung, D.-K., Lee, Y., Park, S.G., Park, B.C., Kim, G.-H., and Rhee, S. (2006) Structural and functional analysis of PucM, a hydrolase in the ureide pathway and a member of transthyretin-related protein family. Proc. Natl. Acad. Sci. USA 103, 9790-9795.
21. Kim, S.-H., Heo, K., Chang, Y.J., Park, S.-H., Rhee, S., and Kim, S.U. (2006) Cyclization mechanism of amorpha-4,11-diene synthase, a key enzyme in artemisinin biosynthesis. J. Nat. Prod. 69, 758-762.
20. Kim, K., Kim, H.-Y., Oh, D.-K., Cheong, J.-J., and Rhee, S. (2006) Expression, purification, and crystallization of D-psicose 3-epimerase from Agrobacterium tumefaciens. J. Microbiol. Biotechnol. 16, 647-650.
19. Kim, K.-Y., Rhee, S..*, and Kim, S.-I.* (2005) Role of the N-terminal domain of endoinulinase from Arthrobacter sp. S37 in regulation of enzyme catalysis. J. Biochem. 138, 27-33.
18. Kim, J.-K., Yang, I.-S., Rhee, S., Dauter, Z., Lee, Y.-S., Park, S.-S., and Kim, K.-H. (2003) Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation. Biochemistry 42, 4084-4093.
17. Umland, T. C., Taylor, K. L., Rhee, S., Wickner, R. B., and Davies, D. R (2001) The crystal structure of the nitrogen regulation fragment of the yeast prion protein Ure2p. Proc. Natl. Acad. Sci. USA 98, 1459-1464
16. Miles, E. W., Fan., Y.-X., Jhee, K.-H., Ro, H. S. McPhie, P., Rhee, S.., and Davies, D. R. (2000) Structure and function of tryptophan synthase, in Biochemistry of Vitamin B6 and PQQ (Martinez-Carrion, M., ed) pp 145-150 Birkhauser Verlag, New York
15. Kwon, T. H., Rhee, S., Lee, Y. S. Park, S. S., and Kim, K. H. (2000) Crystallization and preliminary X-ray diffraction analysis of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas sp. GK1615. J. Struct. Biol. 131, 79-81.
14. Gillette, W. K., Rhee, S., Martin, R. G., and Rosner, J. L. (2000) Structural homology between MarA of the AraC family of transcriptional activator and the integrase family of site-specific recombinases. Mol. Microbiol. , 35, 1582-1583.
13. Han, J., Rhee, S., Miles, H.T., and Davies, D.R. (2000) Crystallization and preliminary crystallographic study of a triple helical DNA. Acta Cryst. D 56, 104-105.
12. Rhee, S., Han, J., Miles, H.T., and Davies, D.R. (1999) Structure of a triple helical DNA with a triplex-duplex junction. Biochemistry, 38, 16810-16815.
11. Martin, R. G., Gillette, W. K., Rhee, S., and Rosner, J. L. (1999) Structural requirements for Marbox function in transcriptional activation of mar/sox/rob regulon promoters in E. coli: sequence, orientation, and spatial relationship to the core promoter. Mol. Microbiol., 34, 431-437.
10. Miles, E. W., Rhee, S., and Davies, D. R. (1999) The molecular basis of substrate channeling. J. Biol. Chem. 274, 12193-12196. (Mini-review)
9. Rhee, S., Martin, R. G., Rosner, J. L., and Davies, D. R. (1998) A Novel DNA-binding motif of MarA: the first structure for an AraC family transcriptional activator. Proc. Natl. Acad. Sci. USA 95, 10413-10418.
8. Rhee, S., Miles, E. W., Mozzarelli, A., and Davies, D. R. (1998) Cryo-crystallography and microspectrophotometry of a mutant (αD60N) tryptophan synthase α2β2 complex reveals allosteric roles of αAsp60. Biochemistry 37, 10653-10659.
7. Rhee, S.., Miles, E. W., and Davies, D. R. (1998) Cryo-crystallography of a truesubstrate, indole-3-glycerol phosphate, bound to a mutant (αD60N) tryptophan synthase α2β2 complex reveals the correct orientation of active site αGlu49. J. Biol. Chem. 273, 8553-8555.
6. Yang, L-h., Ahmed, S. A., Rhee, S., and Miles, E. W. (1997) Importance of conserved and variable C-terminal residues for the activity and thermal stability of the β-subunit of tryptophan synthase. J. Biol. Chem. 272, 7859-7866.
5. Rhee, S., Parris, K. D., Hyde, C., Ahmed, S. A., Miles, E. W., and Davies, D.R. (1997) Crystal structures of a mutant tryptophan synthase α2β2 complex with ligands bound to the active site of the α- and β-subunits reveals ligand-induced conformational change. Biochemistry 36, 7664-7680.
4. Rhee, S., Silva, M. M., Hyde, C. C., Rogers, P. H., Metzler, C. M., Metzler, D. M., and Arnone, A. (1997) Refinement and comparisons of the crystal structures of pig cytosolic aspartate aminotransferase and its complex with 2-methylaspartate. J. Biol. Chem. 272, 17293-17302.
3. Rhee, S., Parris, K. D., Ahmed, S. A., Miles, E. W., and Davies, D. R. (1996) Exchange of K or Cs for Na induces local and long-range changes in the three-dimensional structure of the tryptophan synthase α2β2 complex. Biochemistry 35, 4211-4221.
2. Morino, Y., Tanase, S., Pan., Q.W., Hamasaki, N., Mayumi, M., Rhee, S., Rogers, P. H., and Arnone, A. (1994) in Biochemistry of Vitamin B6 and PQQ (Morino, G., Sannia, G., & Bossa, F. eds) pp 49-53, Basel, Birkhauser
1. Pan, Q.-W., Tanase, S., Fukumoto, Y., Nagashima, F., Rhee, S., Rogers, P. H., Arnone, A., and Morino, Y. (1993) The Functional Role of Val37 and Gly38 in the Mobil Loop of Aspartate Aminotransferase. J. Biol. Chem. 268, 24758-24765.27. 35.